Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSQSTGAPSLFDDGQIGTGGNNNSQSQVKLQAINLIDLLHDGFYLIFLIRNQYVPADPQRFREKILDLLNRFEQQAKKLQFSADDIHDAKYAFCALIDETIVTQQDPSYFNLQNSWLISPLQLSLFGSQLAGYQFFEILEQLRSRGKDRLAALEVFHYCLLLGFQGKYRIESIESLNHLVARVGDEIDYLKGKKVAFSPFSAIPDQIRNIIHRELPFFWILIFLLIFALLTFAGLRFMLNNQNDKALSNYQNVISAPQEQAHITIYLP 2FZ0 Chain:A ((106-141)) ------------------------------------------IRILSGLQEYESNATNELLSSHVGQILDSFHEELVE----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2FZ0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 77 -8935 -116.03 -248.18 target 2D structure prediction score : 0.44 Monomeric hydrophicity matching model chain A : 0.50 3D Compatibility (PKB) : -116.03 2D Compatibility (Sec. Struct. Predict.) : 0.44 1D Compatibility (Hydrophobicity) : 0.50 QMean score : 0.697

(partial model without unconserved sides chains): PDB file : Tito_2FZ0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2FZ0-query.scw PDB file : Tito_Scwrl_2FZ0.pdb: