Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMNSSTEQIISNSLSLRLKQETAAEHERMHQLMSE---AKVFSSKEKYAQFTLSQYYFQLEIEHLFEKEGVAGL--IPDLDIRGRSKQALADLNDLGIQPNGQ-----Q---------LQSENVQLPEALGWIYVSE-GSTLGAAFLFKEAQKHLGFSETFAARNLAAYPEGRAKVWKRFV----KALDEAGFDQTQQDRVVQGALDAFGYFGQALDQLDELK 1WOV Chain:A ((2-216)) -----------TNLAQKLRYGTQQSHTLAENTAYMKCFLKGIVEREPFRQLLANLYYLYSALEAALRQHRDNEIISAIYFPELNRTDKLAEDLTYYYGPNWQQIIQPTPCAKIYVDRLKTIAASEPELLIAHCYTRYLGDLSGGQSLKNIIRSALQLPEGEGTAMYEFDSLPTPGDRRQFKEIYRDVLNSLPLDEATINRIVEEANYAFSLNREVMHDLED--

General information: TITO was launched using: RESULT: Template: 1WOV.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 649 19770 30.46 106.29 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : 30.46 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.409

(partial model without unconserved sides chains): PDB file : Tito_1WOV.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1WOV-query.scw PDB file : Tito_Scwrl_1WOV.pdb: