Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIAKLKREKRKDPLWGIILSIIVGSVLMIY--PLSYDISGWRPLFMLMIMLFWVVCQPTWCGVWFAFGMGIFTDLLLD-APLGLNA-LSFVIVTFITRFLIRERRILTFGNLWTIATLVIIAHLAFMWVTQTMGGIHFSIARHWQPLMTSILTWPVVYYCLAKWRI 3P5N Chain:B ((13-113)) ------------KRLITISMLSAIAFVLTFIKFPIPFLPPYLTLDFSDVPSLLATFTFGPVAGIIVALVKNLLNYLFSMGDPVGPFANFLAGASFLLTAYAI-----------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3P5N.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 312 -83879 -268.84 -975.33 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain B : 0.58 3D Compatibility (PKB) : -268.84 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.58 QMean score : 0.354

(partial model without unconserved sides chains): PDB file : Tito_3P5N.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3P5N-query.scw PDB file : Tito_Scwrl_3P5N.pdb: