Modeling by threading (Tito software)
Unconserved sides chains calculation (Scwrl software)
Evaluation (QMean software)



Input alignment information:
Query sequenceMMDLFLNRKSFVVKSLAITVTALMMSGANAATSDKEEIRKLRQEVEALKALVQEQRQVQQQQQQVQQQQQVQLAEVKAQPQPAAAPASPLASLKSKTGADVNLYGFVRGDANYIIEGADNDFADVSKSNGKTQDKLRATAKTTRLGLDFTAPVSGAKVGGKIELDFASSDNDKSENVRIRHAYLTYNNWLFGQTTSNFLSSHAPEMIDFSTNIGGGTTRIPQVRYNYKLAPATQLFVSAEEGNSSATGDSVKYRLPVLTAKVTQGYADGNGNASARAFVENYKSGAGDDKTGWGIAAGTDFKVSDPLKLFADASYVVGNSNYLYGSNSAYTLVNDNIEQNEFVAVQVGGTYKILPNLRSTLAYGAQFADDGTDYAKNNVTANEKVQQAWINFIYTPVKPIDLGVEYVNGKRDTFEGKSYKDNRVGLMAKYSF
2MLH Chain:A ((104-238))---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------ENQENGTFHAVSSLGLSAIYDFQINDKFKPYIGARVAYGHVRHSIDSTKKTIEVTTVPSNAPNGAVT---TYNTDPKTQN-------------DYQSNSIRRVGLGVIAGVGFDITPKLTLDAGYRYHNWGR--LENTRFKTHEASLGVRYRF


General information:
TITO was launched using:
RESULT:

Template: 2MLH.pdb
Alignment : align.pir
Tito was launched with SMD and SCWRL
Tito text output
Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 332 -661 -1.99 -4.89
target 2D structure prediction score : 0.51
Monomeric hydrophicity matching model chain A : 0.59

3D Compatibility (PKB) : -1.99
2D Compatibility (Sec. Struct. Predict.) : 0.51
1D Compatibility (Hydrophobicity) : 0.59
QMean score : -0.015

(partial model without unconserved sides chains):
PDB file : Tito_2MLH.pdb:





(Unconserved sides chains are recalculated) :
Sequence: align-2MLH-query.scw
PDB file : Tito_Scwrl_2MLH.pdb: