Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYQINALNPKDEGHKARKRFGQNFLHDQRVIAKIVRSVNPRTGDNIVEIGPGLAALTSPLIGE-CDALTVVELDRDLAAGLPERVPHPERLTIVEADALKYDFSQLVKDGRPLRVVGNLPYNISTPLLFHLLEFGSQVKDMHFMLQKEVVERITAEPNTKEYGRLSVMIQYYCQPTFLFEVPAGAFNPPPKVTSAVFRLVPYEQKPITAKDEKALARLVAHVFTQRRKTLRNSLKGMLAEDGFEKAGVDPMARPETLTLAEFVALADQMVA 3FTF Chain:A ((4-240)) --------------RLKKSFGQHLLVSEGVLKKIAEELNIEEGNTVVEVGGGTGNLTKVLLQHPLKKLYVIELDREMVENL--KSIGDERLEVINEDASKFPFCSL---GKELKVVGNLPYNVASLIIENTVYNKDCVPLAVFMVQKEVAEKLQGKKDT---GWLSVFVRTFYDVNYVMTVPPRFFVPPPKVQSAVIKLVKNEKFPV--KDLKNYKKFLTKIFQNRRKVLRKK----IPEELLKEAGINPDARVEQLSLEDFFKL------

General information: TITO was launched using: RESULT: Template: 3FTF.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1193 -21936 -18.39 -92.95 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -18.39 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.500

(partial model without unconserved sides chains): PDB file : Tito_3FTF.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3FTF-query.scw PDB file : Tito_Scwrl_3FTF.pdb: