Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRKVIIDCDPGIDDTLALSLAVKSPDIEVVAITVVCGNVPADIGTQNVLKCLERCDRLDIPVYQGMEKPLKQPFISAQDTHGMDGLGETNF-PMILRKQAE--PLHAVDFLADYFKEKTNTSVIALGPLTNIASALKVNPNIGKHMERFVSMGGTYKSHGNCSPVAEYNYWCDPEAASYVFENLKQTIEMVGLDVTREIVLTPTILEYCCQMNPEEGEYLKAITRFYFDFHWKQERILGCVINDPLAVAYFIEEAIC-EGFKSFTAVE-TQGISRGQTLVDRYEF---WQKPANSKIMTKVDTRLFFRKFLTVLLNAQEETIMKDLERLKMG 3T8J Chain:A ((1-308)) MRHFIIDCDTAEDDVLSLYLLLKN-NIDVVAVTIVEGNISYEQEVKNALWALEQVNR-EIPVYPGANKPLLKNYITVEKVHGKGGIGDVTVEPKRLKAQEKHAALAIIDLANEYAGE---LEFLAISPLTNLALAYLLDNSIVKKIKKVWVMGGAVFGIGNITPVAEFNIWVDPDAAKIVF-NAGFDITMIPWDVIINYPVTDEEWNVIKNMKTRMSELYVSMYLHYRQYSSTVQKINGHPHPDAITTAIAIDGSIATRREKRFVVIDNTDNITRGMTLVDRFDADTSWSDKPNAEIVYEINKKSFMEKIYDLL------------------

General information: TITO was launched using: RESULT: Template: 3T8J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1845 -100158 -54.29 -333.86 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.79 3D Compatibility (PKB) : -54.29 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.79 QMean score : 0.372

(partial model without unconserved sides chains): PDB file : Tito_3T8J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3T8J-query.scw PDB file : Tito_Scwrl_3T8J.pdb: