Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence RPYHGRIQDLRRPLLCDVYIEYPSGQVFDQALGHDIVPAGETAEISGFKCKTVRGTCVILPTCDTPPEYRVTSWKFEREMSDPERTRFIARFPNVRI 3ZTG Chain:A ((1-92)) GPLGSEDDPIPDELLCLICKDIMTDAVVIPCCGNSYCDECIRTALLE----SDEHTCPTCHQNDVSPDALIANKFLRQAVNNFKNETGYTKRLRKQ-

General information: TITO was launched using: RESULT: Template: 3ZTG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 329 -33500 -101.82 -364.13 target 2D structure prediction score : 0.39 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : -101.82 2D Compatibility (Sec. Struct. Predict.) : 0.39 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.014

(partial model without unconserved sides chains): PDB file : Tito_3ZTG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZTG-query.scw PDB file : Tito_Scwrl_3ZTG.pdb: