Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ACSIELLNVNQAVVDTKCIPFDTTTAMKDPTGPGGGQLYKVHVNNLCGIGLDDGQELANGASLRKLSSP 4XHJ Chain:A ((271-329)) ---IELIVVPHTV-KLNAVTSDTTWFQLNPPGPDPGPSYRVY---LLGRGLD-----------------

General information: TITO was launched using: RESULT: Template: 4XHJ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 118 -5571 -47.21 -123.79 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -47.21 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.587

(partial model without unconserved sides chains): PDB file : Tito_4XHJ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4XHJ-query.scw PDB file : Tito_Scwrl_4XHJ.pdb: