Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPGRSCVALVLLAAAVSCAVAQHAPPWTEDCRKSTYPPSGPTYRGAVPWYTINLDLPPYKRWHELMLDKAPVLKVIVNSLKNMINTFVPSGKIMQVVDEKLPGLLGNFPGPFEEEMKGIAAVTDIPLGEIISFNIFYELFTICTSIVAEDKKGHLIHGRNMDFGVFLGWNINNDTWVITEQLKPLTVNLDFQRNNK--------------TVFKA--SSFAGYVGMLTGFKPGLFSLTLNERFSINGGYLGILEWILGKKDVMWIGFLTRTVLENSTSYEEAKNLLTKTKI---------LAPAYFILGGNQSGEGCVITRDRKESLDVYELDAKQGRWYVVQTNYDR----------WKH----------------------------P--FFLDDRRTPAKMCLNRTS---QENISFETMYDVLSTKP----VL------NKLTVYTTLIDVTKGQFETYLRDCPDPCIGW 2HF0 Chain:B ((1-291)) ----------------------------------------------------------------------------------------------------------------------------------------------CTGVRFSDDEGNTYFGRNLDWSFSYG---------------ET--I-LVTPRGYHYDTVFGAGGKAKPNAVIGVGVVMADRPMYFDCANEHGLAIAGLNFPGY-ASF---VHEPVEGTENVATFEFPLWVARNFDSVDEVEETLRNVTLVSQIVPGQQESLLHWFIG-DGKR-SIVVEQMADGM-HVHH-----D-DVDVLTNQPTFDFHMENLRNYMCVSNEMAEPTSWGKASLTAWGAGVGMHGIPGDVSSPSRFVRVAYTNAHYPQQNDEAANVSRLFHTLGSVQMVDGMAKMGDGQFERTLFTSGYSSKTNTYYMNTY---------

General information: TITO was launched using: RESULT: Template: 2HF0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 999 -11461 -11.47 -53.81 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain B : 0.56 3D Compatibility (PKB) : -11.47 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.353

(partial model without unconserved sides chains): PDB file : Tito_2HF0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2HF0-query.scw PDB file : Tito_Scwrl_2HF0.pdb: