Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEQMTLRGTLKGHNGWVTQIATTPQFPDMILSASRDKTIIMWKLTRDETNYGIPQRALRGHSHFVSDVVISSDGQFALSGSWDGTLRLWDLTTGTTTRRFVGHTKDVLSVAFSSDNRQIVSGSRDKTIKLWN-TLGVCKYTVQDESHSEWVSCVRFSPNSSNPIIVSCGWDKLVKVWNLANCKLKTNHIGHTGYLNTVTVSPDGSLCASGGKDGQAMLWDLNEGKHLYTL--DGGDIINALCFSPN-RYWLCAATGPSIKIWDL-EGKIIVDELKQEVISTSSKAEPPQCTSLAWSADGQT-LFAGYTDNLVRVWQVTIGTR 2XL3 Chain:A ((32-288)) ------------------------------------------------KPNYAL-KFTLAGHTKAVSSVKFSPNGEWLASSSADKLIKIWGAYDGKFEKTISGHKLGISDVAWSSDSNLLVSASDDKTLKIWDVSSGKCLKTLK--GHSNYVFCCNFNPQSN--LIVSGSFDESVRIWDVKTGKCLKTLPAHSDPVSAVHFNRDGSLIVSSSYDGLCRIWDTASGQCLKTLIDDDNPPVSFVKFSPNGKYILAATLDNTLKLWDYSKGKCLK-------TYTGHKNEK-YCIFANFSVTGGKWIVSGSEDNLVYIWNL-----

General information: TITO was launched using: RESULT: Template: 2XL3.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1412 -24275 -17.19 -96.71 target 2D structure prediction score : 0.66 Monomeric hydrophicity matching model chain A : 0.73 3D Compatibility (PKB) : -17.19 2D Compatibility (Sec. Struct. Predict.) : 0.66 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.505

(partial model without unconserved sides chains): PDB file : Tito_2XL3.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2XL3-query.scw PDB file : Tito_Scwrl_2XL3.pdb: