Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAFRQALQLAACGLAGGSAAVLFSAVAVGKPRAGGDAEPRPAEPPAWAGGARPGPGVWDPNWDRREPLSLINVRKRNVESGEEELASKLDHYKAKATRHIFLIRHSQYHVDG----SLEKDRTLTPLGREQAELTGLRLASLG-----LKFNKIVHSSMTRAIETTDIISRHLPG-----VCKVSTDLLREGA-------PIEPDPP------------VSHW---KPEAVQYYEDGARIEAAFRNYIHRADAR--QEEDSYEIFICHANVIRYIVCRALQFPPEG--------------------------------WLRLSLNNGSITHLVIRPNG--RVALRTLGDTGFMPPDKITRS 3F3K Chain:B ((2-263)) ---------------------------------------------------------------------------------------------PSLTPRCIIVRHGQTEWSKSGQYTGLTDLPLTPYGEGQMLRTGESVFRNNQFLNPDNITYIFTSPRLRARQTVDLVLKPLSDEQRAKIRVVVDDDLREWEYGDYEGMLTREIIELRKSRGLDKERPWNIWRDGCENGETTQQIGLRLSRAIARIQNLHRKHQSEGRASDIMVFAHGHALRYFAAIWFGLGVQKKCETIEEIQNVKSYDDDTVPYVKLESYRHLVDNPCFLLDAGGIGVLSYAHHNIDEPALELAGPFVSPPE------

General information: TITO was launched using: RESULT: Template: 3F3K.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 863 -8464 -9.81 -44.54 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain B : 0.64 3D Compatibility (PKB) : -9.81 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3F3K.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3F3K-query.scw PDB file : Tito_Scwrl_3F3K.pdb: