Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WYVSYITRPEAEAALRKINQDGTFLVRDSSKKTTTNPYVLMVLYKDKVYNIQIRYQKESQVYLLGTGLRGKEDFLSVSDIIDYF 2EOB Chain:A ((21-95)) WYYDRLSRGEAEDMLMRIPRDGAFLIR---KREGTDSYAITFRARGKVKHCRI--NRDGRHFVLGTSAY----FESLVELVSYY

General information: TITO was launched using: RESULT: Template: 2EOB.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 251 -56064 -223.36 -747.52 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.81 3D Compatibility (PKB) : -223.36 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.81 QMean score : 0.661

(partial model without unconserved sides chains): PDB file : Tito_2EOB.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2EOB-query.scw PDB file : Tito_Scwrl_2EOB.pdb: