Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHY 4DGX Chain:A ((112-222)) WFHGHLSGKEAEKLLTEKGKHGSFLVRESQSHPGDFVLSVRTGDDKGESNDGKSKVTHVMIRCQELKYDVGGGERFDSLTDLVEHY

General information: TITO was launched using: RESULT: Template: 4DGX.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 329 -31887 -96.92 -370.78 target 2D structure prediction score : 0.70 Monomeric hydrophicity matching model chain A : 1.00 3D Compatibility (PKB) : -96.92 2D Compatibility (Sec. Struct. Predict.) : 0.70 1D Compatibility (Hydrophobicity) : 1.00 QMean score : 0.810

(partial model without unconserved sides chains): PDB file : Tito_4DGX.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4DGX-query.scw PDB file : Tito_Scwrl_4DGX.pdb: