Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence WNVGSSNRNKAENLLRGKRDGTFLVRES-SKQGCYACSVVVDGEVKHCVINKTATGYGFAEPYNLYSSLKELVLHY 5DC4 Chain:A ((18-93)) WYHGPVSRNAAEYLLSSGINGSFLVRESESSPGQRSISLRYEGRVYHYRINTASDGKLYVSSESRFNTLAELVHHH

General information: TITO was launched using: RESULT: Template: 5DC4.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 264 -15869 -60.11 -211.59 target 2D structure prediction score : 0.80 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -60.11 2D Compatibility (Sec. Struct. Predict.) : 0.80 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.651

(partial model without unconserved sides chains): PDB file : Tito_5DC4.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5DC4-query.scw PDB file : Tito_Scwrl_5DC4.pdb: