Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ---------MEFFGNK-----------PFTQQPQRAITQANQLLDYKSWSEEDRK-MFSQLHMREEQVLLAQDYA--------LETARAEDLEQGLER-GKVEGRAERKLFTFLDIVRQGLLTSEVASQ----QLGMSVSEFEALL----- 3ZFP Chain:A ((1-148)) MEPLYDKNGAVLFGEPSDTHPQSTLKLPHPRGEKEVIVGIRDLPRKGDCRTGNRLGPVSGLFVKPGP-VFYQDYSGPVYHRAPLEQFKQAPMCEVTKRIGRVTG-SDGNLYH-MYVCTDGCILVKTAKHHHHHVLKWVYNVLDSPIWVTSC

General information: TITO was launched using: RESULT: Template: 3ZFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 403 7928 19.67 72.73 target 2D structure prediction score : 0.27 Monomeric hydrophicity matching model chain A : 0.68 3D Compatibility (PKB) : 19.67 2D Compatibility (Sec. Struct. Predict.) : 0.27 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.179

(partial model without unconserved sides chains): PDB file : Tito_3ZFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3ZFP-query.scw PDB file : Tito_Scwrl_3ZFP.pdb: