Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSFLSKNGAGILACLLISILSWYLGGFFPVIGAPVFAIFIGMLLHPFLSS--YKQLDAGLTFSSKKLLQYAVVLLGFGLNISQVFAVGQSSLPVILSTISIALIIAYLFQR-FFALD--TKLATLVGVGSSICGGSAIAATAPVIHAK--EKEVA-QAISVIFFFNVLAALIFPTLGTWLHLSNEGFALFAGTAVNDTSSVTAAASAWDSLYQSNTLESATIVKLTRTLAIIPITLFLSYWQSRQQENKQSLQLKKVFPLFILYFILASLLTTLLTSLGVSSSFFTPLKQLSKFLIVMAMSAIGLKTNLVAMVKSSGKSILLGAICWIAIILTTLGMQTLIGIF 4D0A Chain:B ((29-175)) ------------------------------IPDIPLLLLLGLIIGPFLQIIPSDSAMEIFEYAGPIGLIFILLGGAFTMRISLLKRVIKTVVRLDTITFLITLLISGFIFNMVLNLPYTSPVGYLFGA---ITAATDPATLIPVFSRVRTNPEVAITLEAESIFNDPLGIVSTSVILGLF--------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 4D0A.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 360 -49169 -136.58 -353.73 target 2D structure prediction score : 0.63 Monomeric hydrophicity matching model chain B : 0.52 3D Compatibility (PKB) : -136.58 2D Compatibility (Sec. Struct. Predict.) : 0.63 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.083

(partial model without unconserved sides chains): PDB file : Tito_4D0A.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D0A-query.scw PDB file : Tito_Scwrl_4D0A.pdb: