Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MFVWYNLFLKKEREVISMRKWTKGFLIFGVVTTVIGFILLFVGIQSDGIKSLLSMSKEPVYDSRTEKLTFGKEVENLEITLHQHTLTITDSFDDQIHISYHPSLSAHHDLITNQNDRTLSLTDKKLSETPFLSSGIGGILHIASSYSSRFEEVILRLPKGRTLKGINISANR-GQTTIIN-ASLENATLNT-NSYILRIEGSRIKNSKLT--TPNIVNIFDTVLTDSQLES-TENHFHAENIQVHGKVELT-AK-DYLRIILDQKESQRINWDISSNYGSIFQFTREKPESRGTELSNPYKTEKTDVKDQLIARSDDNIDLISTPSRR 3JX8 Chain:A ((116-234)) -----------------------------------------------------------------------------------------------------------------------------------------------------------------KVNRMEINMAGSGNVELRGPVKGYKLECNMAGSGNIIAKDIQLDNLSCSLASSGEIEVIG-TVDRASFNVAGSGEIKAFDCQA-RKAECNIASSGEISVYATQI----LDANI-VGSGEIHYKGDP-----------------------------------------

General information: TITO was launched using: RESULT: Template: 3JX8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 654 21750 33.26 195.94 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain A : 0.56 3D Compatibility (PKB) : 33.26 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.56 QMean score : 0.257

(partial model without unconserved sides chains): PDB file : Tito_3JX8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3JX8-query.scw PDB file : Tito_Scwrl_3JX8.pdb: