Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYFPTSSALIEFLILAVLEQGDSYGYEISQTIKLIA---NIKESTLYPILKKLEGNSFLTTYSR--EFQGRMRKYYSLTNGGIEQLLTLKDEWTLYTDTINGIIEGSIRHDKN 2ZFW Chain:A ((41-131)) -----SKELAVCYVLAVLRHEDSYGTELIQHLETHWPNYRLSDTVLYTALKFLEDEQIISGYWKKVEGRGRPRRMYQLAQANDDRSRDLAQLWERY-----------------

General information: TITO was launched using: RESULT: Template: 2ZFW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 290 -1370 -4.72 -15.93 target 2D structure prediction score : 0.53 Monomeric hydrophicity matching model chain A : 0.72 3D Compatibility (PKB) : -4.72 2D Compatibility (Sec. Struct. Predict.) : 0.53 1D Compatibility (Hydrophobicity) : 0.72 QMean score : 0.563

(partial model without unconserved sides chains): PDB file : Tito_2ZFW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2ZFW-query.scw PDB file : Tito_Scwrl_2ZFW.pdb: