Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --MLKRFLALVWL---------RCQIILSNKSILLQVLVPFAFT------------YFYKYLMETQGKVNDQQALVLLMMCLPFSLALAVGSPIT-----------------IILSEEKEKYNLQTLL--LSGVKGSEYILSTMFLPFL-LTF-VIMGTTPLIL--GVTIVHTFNYITIVLLTSLS-IILFYLL-IGLTAKSQVVAQVISLPAMILVAFLPMLSSLDK-TVAKITDYSFMGLFTKFFTKWEGFSWNETLI-PNLTLLI---WIVLLLTLITIT-IRKKKIS 3G3L Chain:A ((10-305)) PAEAKYYIAGTITDATTGQELTTAKVTLGDKSVTSSFNEQVNYKAEGYALVVSADGYYPVKRQVYLNQVSDGQTSVATVNVALVSVEAAVIPPVVPPTDPETDINEGEATKVADKAVEVAKPSESTVTDMLAGTTATPEEKKALDETLEMAGGMKVGETTPEVLADGSILAITPVKFTNPIQDAPAMVPYFYNEGCELTGDVKEVAAPVAVAADIQKAFLSNAAKALNMNAGFVQKIGYTRISVLNGYSILGYTIKGQLVSKKLTFLISGKYYEGIVSYQKSVMIYPNYYS

General information: TITO was launched using: RESULT: Template: 3G3L.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 984 -49644 -50.45 -209.47 target 2D structure prediction score : 0.25 Monomeric hydrophicity matching model chain A : 0.63 3D Compatibility (PKB) : -50.45 2D Compatibility (Sec. Struct. Predict.) : 0.25 1D Compatibility (Hydrophobicity) : 0.63 QMean score : 0.054

(partial model without unconserved sides chains): PDB file : Tito_3G3L.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3G3L-query.scw PDB file : Tito_Scwrl_3G3L.pdb: