Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ------------------------------------MLRLVYYQFLHNKKQWLGVSPVIFVS------SLVMGLAVNGVINVENNSQVFV-------------GLPDPKP---IFMFP-----IV-----------FGGVTLFFVLSNIINMLVEIFRDDYELLEVLGASRLQLSFLVGGQIFIIS-----------SIISFIAYLCS-------------------------IFVTSNYYYFLQYFFGENILPDIQFQTSAVGCIITVVLISFLAFLSGCFYTFKKIRNRKSSKIRHVLSIVKRILLLAGF---------------------------SVIWLLSLQQIFQDSTILAKAQIIFNIVILDIVIIYQLSPFIQSCFIKLLSIIIFRNNFMF-----IVSKWNLLYCKSYI-----------KSISAAITGAILL-ISSFQMISQNILSQFQDDSDLELKVAFIVYVGAPILIVLANIISIAFLSSHQERIEIQQFEILGTSNYQMVKIKVGEAIFLTFVTSLIAFLLNIKIMALIYYSLEDIL---IDDMNLLGLILPNFIVSIILF----ILIFITKSSYFIFKNAKIIS-------------------------------- 5F7C Chain:A ((12-723)) LFDFDTELLRDESLWKACKPTAVYEKDGDICVTVPFQKQLLANDMVADTAVPREEYTLIIRQYNIGITRLFLGFGEYEILFTQDGTKRAVINVEEPALDRWSELLPDPQETLDITLYPDGKREIRLAAYDHFSPPRYDGLPIAFCKRTGKKERATLSFESRPDECFAGTGERFFKMDLSGQTLFLKNQDGQGVNNRRTYKNIPFYLSSRMYGTFYHTCAHSKLSLAGHSTRSVQFLSDQAMLDAFVIAGDTMEEILRGYRDLTGYPSMPPLWSFGVWMSRMTYFSADEVNEICDRMRAEHYPCDVIHLDTGWFRTDWLCEWKFNEERFPAGTIDFTYPKATEWYKGLLKQLLDMGVTCIKTDFGENIHMDAVYKGMKPELLNNLYALLYQKAAYEITKEVTGDGIVWARAAWAGCQRYPLHWGGDSCSSWDGMAGSLKGGLHFGLSGFAFWSHDVPGFHTLPNFMNSIVAEDVYMRWTQFGVFTSHIRYHGTNKR----EPWHYPAIAPLVKKWWKLRYSLIPYIIEQSKLAVESGWPLLQALILHHPEDKLCWHIDDEYYFG---NDFLVAPVMNSENRRDIYLPEGQWVNFFTGERLQGGRWLKEVYVPLEEMPVYVRENAVIPIYPEEV

General information: TITO was launched using: RESULT: Template: 5F7C.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2249 -203610 -90.53 -471.32 target 2D structure prediction score : 0.26 Monomeric hydrophicity matching model chain A : 0.61 3D Compatibility (PKB) : -90.53 2D Compatibility (Sec. Struct. Predict.) : 0.26 1D Compatibility (Hydrophobicity) : 0.61 QMean score : 0.120

(partial model without unconserved sides chains): PDB file : Tito_5F7C.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5F7C-query.scw PDB file : Tito_Scwrl_5F7C.pdb: