Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNIVLLFLFSGGKMAKELQSSCYIVISFLVREMGIDIVEAISLMAELEKSGLVRLESSGDLILKELGGAL 3C16 Chain:A ((106-121)) -------------------------------EMGMDMIEAISLVREM-----------------------

General information: TITO was launched using: RESULT: Template: 3C16.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 2 -525 -262.25 -32.78 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain A : 0.52 3D Compatibility (PKB) : -262.25 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.52 QMean score : 0.480

(partial model without unconserved sides chains): PDB file : Tito_3C16.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3C16-query.scw PDB file : Tito_Scwrl_3C16.pdb: