Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MPIDTQLTTLFCLIDDFCTDITQNVEQYMLTYGQTKRLRQSKIHASEVITLLLWFHLTGSRNFKAFYLYWAKPFLCSYFTNLPSYSRFIELKAKYAMYFVALIESLKVHSAGIAFIDSTKLAVCHNKRIHQHRVFADSASRGKTSVDWFYGFKIHLICDHIGRLVSYCITTGNVDDRKVLPDLIEHSKLKGKLFGDRSYVGKNWKSRLAEVGVQLITRVKRNMKPQVLAPFDHAVLRKR----GIIEAPFKLMKSQFDLEHSRHRSKMGLLTTIFAALTLYALVLVNGYKSGIQQILKPIDLNSA 1AZS Chain:C ((128-221)) --------------------------------------------------------------------------------------------------------------------------------------------------------------------VDYILSVMNVPDFDFPPEFYEHAKALWEDEGVRACYERSNEYQLIDCAQYFLDKIDV-IKQDDYVPSDQDLLRCRVLTSGIFETKFQVDKVNFHM----------------------------------------------

General information: TITO was launched using: RESULT: Template: 1AZS.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain C - contact count / total energy / energy per contact / energy per residue : 140 -18045 -128.89 -200.50 target 2D structure prediction score : 0.41 Monomeric hydrophicity matching model chain C : 0.54 3D Compatibility (PKB) : -128.89 2D Compatibility (Sec. Struct. Predict.) : 0.41 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.184

(partial model without unconserved sides chains): PDB file : Tito_1AZS.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1AZS-query.scw PDB file : Tito_Scwrl_1AZS.pdb: