Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTANIMTTLKNQITDALKTSMRAKDMATVTVLRSL---------QAAIKQIEVDERIELDDAQVLAVIEKQIKQR------KESIKAFEGAGRDDLASKEQAEAEVLSQFLPEAMTEEELDSLIEQTIAAQEATSMKDMGKVMNSLRPIIAGRADPAQVSAKIKAKLA 3SG2 Chain:A ((337-430)) -----------------------------TVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKYTDSEEEIREAFRVFDKDGNGYISA---AELRHVMTNLGEKLTDEEVDEMIRE------------------------------------------

General information: TITO was launched using: RESULT: Template: 3SG2.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 125 9776 78.21 123.75 target 2D structure prediction score : 0.76 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 78.21 2D Compatibility (Sec. Struct. Predict.) : 0.76 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.470

(partial model without unconserved sides chains): PDB file : Tito_3SG2.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3SG2-query.scw PDB file : Tito_Scwrl_3SG2.pdb: