Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNEIEMFCELLSLLGLKVV-------PKDYQSID-KERVAALLVMSKSWMNRIETVDDLFHDEISCQKEKLGY 1LFP Chain:A ((179-237)) --ELYEVKENLEKLGVPIEKAQITWKPISTVQINDEETAQKVIKLLN-ALEELDDVQQVIAN-----------

General information: TITO was launched using: RESULT: Template: 1LFP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 32 -2149 -67.16 -42.14 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -67.16 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_1LFP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1LFP-query.scw PDB file : Tito_Scwrl_1LFP.pdb: