Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MHEITLHEVRQLIASLRTVYAAQFNKQFPTSGESAIPLSVVEQIALKTLVGVQQNQFNNALARLLTAGGRFMPSFAEFRTWCIGESWMSPEEAWSRACKFTTDRTVVITQITKYALDEVMYLIEAGQMRAAQDNFFGTYNVMVAKAQLKGRQQEFYTPPLQLEHKEPEHTPVSNDEVQKQLKSLME-RLKINGRKPAPVQKLQAKEKEPELAKELGPDPFDNPHEYAEMCRREGMPIPRNILQLIEGANV 2IQC Chain:A ((12-210)) EDSLMKTQAELLLERLQEVRP--------------------ARFLSSLWERLPQNNFLKVIAVALLQPGS-----QVLVHWLLGN-----SEVFAAFCRA---------------LPAGLLTLVTSRHPALSPVYLGLLTDWGQRLHYDLQKGIWVGT---------ESQDVPWEELHNRFQSLCQAPPPLKDKVLTALETCKAQDGDFEV---------------------PGLSIWTDLLLALRSG--

General information: TITO was launched using: RESULT: Template: 2IQC.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 652 -75747 -116.18 -440.39 target 2D structure prediction score : 0.65 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -116.18 2D Compatibility (Sec. Struct. Predict.) : 0.65 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.487

(partial model without unconserved sides chains): PDB file : Tito_2IQC.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2IQC-query.scw PDB file : Tito_Scwrl_2IQC.pdb: