Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMSSVSIAEYRKLFPIKKNKKRRSAKQVARQLSVGEMVLATHLRACKIGFEQEYKFHPKRKWRADFLITGTKILVEVEVGIWSGGRHT---------------RGKGYLGDMEKYNSAAMMGFTVLRFSTEQVKSGV------AIKQIEQLVG 1VSR Chain:A ((3-118)) ---------------------------------AIEKRLASLLTGQGLAFRVQDAS---LPGRPDFVVDEYRCVIFTHGCFWHHHHCYLFKVPATRTEFWLEKIGKNVERDRRDISRLQELGWRVLIVWECALRGREKLTDEALTERLEEWI-

General information: TITO was launched using: RESULT: Template: 1VSR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 289 -24903 -86.17 -262.14 target 2D structure prediction score : 0.55 Monomeric hydrophicity matching model chain A : 0.64 3D Compatibility (PKB) : -86.17 2D Compatibility (Sec. Struct. Predict.) : 0.55 1D Compatibility (Hydrophobicity) : 0.64 QMean score : 0.416

(partial model without unconserved sides chains): PDB file : Tito_1VSR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1VSR-query.scw PDB file : Tito_Scwrl_1VSR.pdb: