Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence --------------------MVMLSLSVFFKGLGIGSGLIVA-IGAQNAFVLKQG-------LKQQYVFWLCLIC---ALSDSILIACGVL---------------------GFAEIM--TASPILITVAKYLGATFLFVYGAKAFYAAFKTNQGMELDSSQKQTLTQALITCLAFTWLNPHVYLDTI--VLIGSVATQLEDKISFALGSILASWIFFFSLGYGARFL--KPLFINPKAWKILDFMIGCVMWSIAISLLI------------------- 5MUW Chain:A ((2-276)) PIVVTQAHIDRVGIAADLLDASPVSLQVLGRPTAINTVVIKTYIAAVMELASKQGGSLAGVDIRPSVLLKDTAIFTADVESDVDVLDTGIYSVPGLARKPVTHRWPSEGIYSGVTALMGATGSGKSITLNEKLRPDVLIRWGEVA-EAYDELDTAVHIS-----TLDEMLIVCIGLGALGFNVAVDSVRPLLFRLKGAASAGGIVAVFYSLLTDISNLFTQYDCSVVMVVNPMVDAEK----IEYVFGQVMASTVGAILCADGNVSRTMFRTNKGRIFN

General information: TITO was launched using: RESULT: Template: 5MUW.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 778 -88329 -113.53 -460.05 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -113.53 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.249

(partial model without unconserved sides chains): PDB file : Tito_5MUW.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5MUW-query.scw PDB file : Tito_Scwrl_5MUW.pdb: