Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKISLSSFQIGSFFALCSAFLFSTKAIFIK--QTYALSPLVDGTVLMALRMLSALPFFLLICWFNRHHNQGIKKKDWLILIFAGLLGYYFASWLDFMSLMF--ISASLERIILFLYPTLTVIASSLLYKQKLDVKSLFAIFLSYGGTVLV----MLQEHNNATIQGKFWLGTSFVFASALAFAG-YLLLTPPLIKKFGSWNFTGLALTVACIGTLTHYTLSTSHPFQLLLQLPSSVIWYGIGLGFLVTVLPTVMLMQSIERLGASQSAMIASIGPVLTILLAVAFLNEHLNIWQWVGCF-LNIIGVMMITLRKK-KLKH 2QVP Chain:A ((8-273)) QTFVWRSEIFECQSTDIQRFYSLLAIETERLGLGSKILGQAGHHPLYLLQS------------------PGQKAGLPNLLISAGFHGEESAGPWGLLHFLSQLDGELFKRVNLSVLPLVNPTGFAKGHRFNELGENPNRGFFIENGKAKPGADTSAEGRILLEHAHLLQVASRDGILTCHEDVLMTDTYVYTFEPSQAP---GRFSHSLRDALGQYFPIAADGDVDN--CPVRSGVIFNHFDTS---------------------FESFLVRSGARVGCCSETPGQQPLDQRILANAAAMNTFV---------NMLAPE

General information: TITO was launched using: RESULT: Template: 2QVP.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1548 -104382 -67.43 -409.34 target 2D structure prediction score : 0.36 Monomeric hydrophicity matching model chain A : 0.54 3D Compatibility (PKB) : -67.43 2D Compatibility (Sec. Struct. Predict.) : 0.36 1D Compatibility (Hydrophobicity) : 0.54 QMean score : 0.244

(partial model without unconserved sides chains): PDB file : Tito_2QVP.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2QVP-query.scw PDB file : Tito_Scwrl_2QVP.pdb: