Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MYYPLLSIALGSVLGAWLRWFLGLKLNPIYPQIPLGTVTVNLVGGFIIGFAMAYFAHSDLSPNYKLFVITGFCGALTTFSTFSIEIVTLLQSGKWGMAMLAISIHLIGSLIFTCLGLATYYWVAGH 5UIG Chain:A ((48-160)) ---------LGNVLVCWAVW--------------LNSNLQNVTNYFVVSLAAADIAVGVLAIPFAITISTGFCAACHGCLFIACFVLVLTQSSIFSLLAIAIDRYIAIRIPLRYNGLVT-------

General information: TITO was launched using: RESULT: Template: 5UIG.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 226 -50397 -223.00 -524.97 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : -223.00 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.063

(partial model without unconserved sides chains): PDB file : Tito_5UIG.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UIG-query.scw PDB file : Tito_Scwrl_5UIG.pdb: