Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQDIIAYFIQNLTALYIAVALVSLCIGSFLNVVIYRTPKMMEQDWQQECQMLLNPEQPIIDHEKLTLSKPASSCPACQQPIRWYQNIPVISWLTLRGKCGHCQHPISIRYPAVELLTMLCSLVVVMVFGPTIQMLFGLVLTWVLIALTFIDFDTQLLPDRFTLTLAALGLGINTFNIYTSPNSAIWGYLIGFLCLWIVYYLFKVITGKEGMGYGDFKLLAALGAWMGPLMLPLIVLLSSLLGAIIGIILLKLRNDNQPFAFGPYIAIAGWVAFLWGDQIMKIYLGG 2Y0S Chain:P ((4-34)) -----------------------------------------------------------------------YRCGKCWKTFTDEQLK---V--LPGVRCPYCGYKII-----------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 2Y0S.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain P - contact count / total energy / energy per contact / energy per residue : 81 -8555 -105.61 -275.95 target 2D structure prediction score : 0.45 Monomeric hydrophicity matching model chain P : 0.36 3D Compatibility (PKB) : -105.61 2D Compatibility (Sec. Struct. Predict.) : 0.45 1D Compatibility (Hydrophobicity) : 0.36 QMean score : 0.616

(partial model without unconserved sides chains): PDB file : Tito_2Y0S.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y0S-query.scw PDB file : Tito_Scwrl_2Y0S.pdb: