Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKALPIAVAVVLGGAALVPVYYATQHPTTEVGHKADKNASPIQKISYVLGYEVAQQTPPELDTKAFVQGIHDVRNKQPSAYTQEDLKAAVAAYEKELQQKMQHQDKPEQAGTATDSADAQFLAENKTKAGVKTTASGLQYIITKEGTGKQPTAQSIVKVHYEGRLINGQVFDSSYKRGQPVEFPL--NQVIPGWTEGLQLMKEGGKATFFIPSNLAYGPQELPG-IPANSTLIFDVELISVK 2Y78 Chain:A ((23-133)) -----------------------------------------------------------------------------------------------------------------------------------VVTTESGLKYEDLTEGSGAEARAGQTVSVHYTGWLTDGQKFDSSKDRNDPFAFVLGGGMVIKGWDEGVQGMKVGGVRRLTIPPQLGYGARGAGGVIPPNATLVFEVELLDV-

General information: TITO was launched using: RESULT: Template: 2Y78.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 532 -12158 -22.85 -112.57 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.67 3D Compatibility (PKB) : -22.85 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.67 QMean score : 0.678

(partial model without unconserved sides chains): PDB file : Tito_2Y78.pdb: (Unconserved sides chains are recalculated) : Sequence: align-2Y78-query.scw PDB file : Tito_Scwrl_2Y78.pdb: