Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRYILIALLIIVFGYSLALVLQNPTELPVDLLFTQVPAMRLGLLLLLTLALGIVVGLLLGVQVFRVFQKSWEIKRLRKDIDHLRKEQIQSAQLAAAEAAANVRHEKTVVDIYPQDKNSTPL 1KD8 Chain:A ((1-35)) -----------------------------------------------------------------------EVKQLEAEVEEIESEVWHLENEVARLEKENAECEA---------------

General information: TITO was launched using: RESULT: Template: 1KD8.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 5 -274 -54.80 -7.83 target 2D structure prediction score : 0.89 Monomeric hydrophicity matching model chain A : 0.44 3D Compatibility (PKB) : -54.80 2D Compatibility (Sec. Struct. Predict.) : 0.89 1D Compatibility (Hydrophobicity) : 0.44 QMean score : 0.905

(partial model without unconserved sides chains): PDB file : Tito_1KD8.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1KD8-query.scw PDB file : Tito_Scwrl_1KD8.pdb: