Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAKDTRTRKKVTRTVSEGVAHIHASFNNTIVTITDRQGNALAWATSGGQGFRGSRKSTPFAAQVAAEVAGKAALDYGLKNLDVLVKGPGPGRESAVRALGAVGYKINSITDVTPIPHNGCRPPKKRRV 1HR0 Chain:K ((12-126)) ------------RQVASGRAYIHASYNNTIVTITDPDGNPITWSSGGVIGYKGSRKGTPYAAQLAALDAAKKAMAYGMQSVDVIVRGTGAGREQAIRALQASGLQVKSIVDDTPVPHNGCRPKKKFR-

General information: TITO was launched using: RESULT: Template: 1HR0.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain K - contact count / total energy / energy per contact / energy per residue : 555 -37483 -67.54 -325.93 target 2D structure prediction score : 0.74 Monomeric hydrophicity matching model chain K : 0.86 3D Compatibility (PKB) : -67.54 2D Compatibility (Sec. Struct. Predict.) : 0.74 1D Compatibility (Hydrophobicity) : 0.86 QMean score : 0.584

(partial model without unconserved sides chains): PDB file : Tito_1HR0.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1HR0-query.scw PDB file : Tito_Scwrl_1HR0.pdb: