Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSKRIIQSVLSVSVLASMMSMAFAAQNEQEQAEQTLEKPAEPVKLETIFVTAE--EQVKQSL--GVSVITKEDLAKLPVRNDISDYVRRMPGVNLTGNSATGQRGNNRQIDIRGMGPENTLILVDGKPINSRNSVRYGWKGERDTRGDSNWVPAEAIESIEVLRGPAAARYGSGAAGGVVNIITKKVTNETHGSVEFYTSQPEDSKEGSSNRVGFNVSG-PLIKDVLSYRLYGNYNKTEADDVDINKSIGSTA-AGREGVKNKDISGRLAWQATDQQTVLLDVSSSKQGNIYSGDSQLNANAEADAILSQLIGKETNTMYRDSY--ALTHEGDWSWGKSKLVAQYDKTHNKRLPEGLAGSVEGKINSLDDKATSRLETLRFNGEANIPFEYYLPQVLTVGTEWVEDRFKDNVSTTQGKDSSGSGYGDQLAKGDRSKMESRIASAYIEDNLKVTDSTDVVLGL------------RFDDHSKSGSNWSPSLNITQKLNDNFTLKGGVAKAYKAPNMYQNAEGYLLSTNGNGCPANIESRCLLQGNGDLKPETSVNKELGIQFQRDIVNASLTWFRNDYKDKIVAGTHVVGTADGSSTNANTGAVTNTKWNILRWENTPKALIQGFEGSLGLDFGDIRWTNNFTYMMDSKDKQTGNPLSLVPIYTINSIFDYDITDQLDVNFVFTQYGRQKSRQFAENRLESGIGSGGANSALKPSTVKSYSTAGINVGYKFSDQISTRVGVSNLFDKQILRDSNSISQTYNEPGRAYYASLKYSF 3M8D Chain:A ((7-594)) ----------------------------------------------TLVCTANRFEQPRSTVLAPTTVVTRQDIDRWQ-STSVNDVLRRLPGVDITQNGGSGQLSS---IFIRGTNASHVLVLIDGVRLNLA-----GVSGSADL----SQFPIALVQRVEYIRGPRSAVYGSDAIGGVVNIITTRDEPGTEISAGW----------GSNSYQNYDVSTQQQLGDKTRVTLLGDYAHTHGYDVVAYGNTGTQAQTDNDGFLSKTLYGALEHNFTDAWSGFV--------RGYGYDNRTNYDAYYSPGSPLL---DTRKLYSQSWDAGLRYNGELI--KSQLITSY--SHSKDY------NYDPHYGRYDSSAT-------------------LDEMKQYTVQWA-----NNVIVGHGSIGAGV---------DWQKQTTTP------DGYDQRNTGIYLTGLQQVGDFTFEGAARSDDNSQFGRHGTWQTSAGWEFIEGYRFIASYGTSYKAPNLGQLYGFY--------------------GNPNLDPEKSKQWEGAFEGLTAGVNWRISGYRNDVSDLIDYDDHT-----------------------LKYYNEGKARIKGVEATANFDTGPLTHTVSYDY-VDARNAITDTPLLRRAKQQVKYQLDWQLYD-FDWGITYQYLGTRYDKDYSSYPYQT-VKMGGV------------SLWDLAVAYPVTSHLTVRGKIANLFDK----DYETV-YGYQTAGREYTLSGSYTF

General information: TITO was launched using: RESULT: Template: 3M8D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 3369 205596 61.03 365.83 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : 61.03 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.252

(partial model without unconserved sides chains): PDB file : Tito_3M8D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3M8D-query.scw PDB file : Tito_Scwrl_3M8D.pdb: