Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MSILVIADHNNQTLNGATLNVVAAAQ--KIGGDITVLVAGSGAQAVADAAA-KVAGVSKVLLADNAAY---ANQLAENVAGLVADLAKGYKYVLAASTTTGKNILPRVAALLDVSMITDIISVESANTFKRPIYAG-NAIATVQSD---EAIIVGTVRGTAFDPVAAEGGSAAVETVGEVKDAGV-SKFVSEEIVKL---DRPELTAARIVVSGGRGVGSGENYHKVLDPLADKLGAAQGASRAAVDAGFVPNDFQVGQTGKIV-APELYIAVGISGAIQHLAGMKDSKVIVAINKDEEAPINSVADYWLVGDLNAVVPELVSKL 3CLR Chain:D ((4-319)) --ILVIAEHRRNDLRPVSLELIGAANGLKKSGEDKVVVAVIGSQADAFVPALSVNGVDELVVVKGSSIDFDPDVFEASVSALIA--AHNPSVVLLPHSVDSLGYASSLASKTGYGFATDVYIVEYQGDELVATRGGYNQKVNVEVDFPGKSTVVLTIRPSVFKPL--EGAGSPV--VSNVDAPSVQSRSQNKDYVEVGGGNDIDITTVDFIMSIGRGIGEETNVEQFRE-LADEAGATLCCSAPIADAGWLPKSRQVGQSGKVVGSCKLYVAMGISGSIQHMAGMKHVPTIIAVNTDPGASIFTIAKYGIVADIFDIEEELKAQL

General information: TITO was launched using: RESULT: Template: 3CLR.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 1651 -10041 -6.08 -33.36 target 2D structure prediction score : 0.58 Monomeric hydrophicity matching model chain D : 0.80 3D Compatibility (PKB) : -6.08 2D Compatibility (Sec. Struct. Predict.) : 0.58 1D Compatibility (Hydrophobicity) : 0.80 QMean score : 0.464

(partial model without unconserved sides chains): PDB file : Tito_3CLR.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3CLR-query.scw PDB file : Tito_Scwrl_3CLR.pdb: