Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MQRQLVSSSRVEQKNWDR------PVLSEWIEQFVNTVQAVQ-TPVQIVAHSFGCLTSVAALAEHPELQLQVKKLILVAPANPARFGE----------------AG------------FA--------R--HSLTD-------------Y---------------------KDYFH-----Q-LKINVPATLLISENDPWLDFFDALQLAKSW-QLTPINLGQVGHINVASGFGP--FPDLLNHLLPEDSMCSHSSIDPVKTHFSLKFA 4Y7D Chain:A ((62-301)) ------RAIVVDRPGYFGTPLGSGVTPDEQAELYAALFDALGLAAAGVLCWSGGGPSSYRLAARHP---DRVRALVSVAAVSHRYHFDGEKGAEKVLMGTGLGRRMLQLMAAHTPEKLVSATIAAEGHLSKEHVAERVAQIMADPDKERFTLELAVSANHSGPRKAGFDNDMDQFARIDSLELDRITAPTLVVSGTADSDVDPEFSRFAAAQIAGSELVHLDAGTHLAFWVHPDSGPVRRRAAELLRAG--------------------

General information: TITO was launched using: RESULT: Template: 4Y7D.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 505 6311 12.50 41.52 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 12.50 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.531

(partial model without unconserved sides chains): PDB file : Tito_4Y7D.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4Y7D-query.scw PDB file : Tito_Scwrl_4Y7D.pdb: