Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MMKKEIVLTAAMMLFSMVASTTFVSATEVYPKEYNTEGTITFEAGDEGVTPPVDPENPDPNK---PVDPSDPPSPG-TGGALSIDYGSKFKFGTQKISTADKTYYAAADVMNDGSRKPTYVQVTDRRSTLSGWKLSVSQPEQFKTASGDELVGAQLKFTKGQAVSLVDPTYTPQTVNSELTLTPGGNNTLAINAKSGTGVGTWVYRFGANENENQDAVQLSVPGKSVKLAQQYSTKLVWTLEDTPNN 1G9J Chain:A ((113-195)) ------------------------------------------------------PEFQDPSKYPSPLDTSQPVGRDPINSQLTSAYGTSMLYGMHWILDVDNWYGFGARA--DGTSKPSYINTFQRGEQESTWE-TIPQP-----------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1G9J.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 151 16998 112.57 215.16 target 2D structure prediction score : 0.67 Monomeric hydrophicity matching model chain A : 0.59 3D Compatibility (PKB) : 112.57 2D Compatibility (Sec. Struct. Predict.) : 0.67 1D Compatibility (Hydrophobicity) : 0.59 QMean score : 0.277

(partial model without unconserved sides chains): PDB file : Tito_1G9J.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1G9J-query.scw PDB file : Tito_Scwrl_1G9J.pdb: