Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKAIRKECCLM---KFFQEFFIIIFFSVLGEGIRIFAHL-----------PIPGSILGILFLFL--AFEMHLVDPEKIG---ATGDFLLNNLAILFVPAGVGLLEYFDDIASIW---PVLLGAVVVCSIVTMVAAGKTAEGVEAL-LGYVRKKKAERVSVNEMEENQID--------------------------------------- 4D10 Chain:G ((12-219)) TGQNQEQFLLLAKSAKGAALATLIHQVLEAPGVYVFGELLDMPNVRELAESDFASTFRLLTVFAYGTYADYLAEARNLPPLTEAQKNKLRHLSVVTLAAKVKCIPYAVLLEALALRNVRQLEDLVIEAVYADVLRGSLDQRNQRLEVDYSIGRDIQRQDLSAIARTLQEWCVGCEVVLSGIEEQVSRANQHKEQQLGLKQQIESEVAN

General information: TITO was launched using: RESULT: Template: 4D10.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain G - contact count / total energy / energy per contact / energy per residue : 560 -22824 -40.76 -156.33 target 2D structure prediction score : 0.56 Monomeric hydrophicity matching model chain G : 0.65 3D Compatibility (PKB) : -40.76 2D Compatibility (Sec. Struct. Predict.) : 0.56 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.266

(partial model without unconserved sides chains): PDB file : Tito_4D10.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4D10-query.scw PDB file : Tito_Scwrl_4D10.pdb: