Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIKKMIYFLGSLITVLSSFIYLKKKKQAEKSEGLSKYQGTWFFSDSQQKQQLQITSDYTFLINGQSLGTSVVEIDEYRLVVRDQYGF-YLIFSYP--------EQTLYDESADTSYKLLSEK 5I21 Chain:B ((25-71)) -----------------------------------------------------------YLVNGIKLQGQIESFDQFVILLKN--TVSQMVWKHAISTVVPSRPVRLP--------------

General information: TITO was launched using: RESULT: Template: 5I21.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 105 -21965 -209.19 -578.03 target 2D structure prediction score : 0.47 Monomeric hydrophicity matching model chain B : 0.57 3D Compatibility (PKB) : -209.19 2D Compatibility (Sec. Struct. Predict.) : 0.47 1D Compatibility (Hydrophobicity) : 0.57 QMean score : 0.633

(partial model without unconserved sides chains): PDB file : Tito_5I21.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5I21-query.scw PDB file : Tito_Scwrl_5I21.pdb: