Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEPKLIDPRNLYHTEKFPKQDQDTPALQQNMIPVPDCGEESYEGNNQLENRRVLITGGDSGIGRAAAIAFAREGADIALQFFPGEEKDAEEVAKYVKDAGRKIVLLPFDLRDEQAPKEIIDKAVSELGGLDTMVLNAGQQISCQS-IAELPLEQVKDTFMINIIAMFALVKEAVPHI--PAGGAIVTTTSVQAFNPSEHLLDYAATKASIANFTIGLAKQLAPKGIRVNGVAPGPIWTPLQLDEGQPSDSIPEFGQQSLLERAGQPGELAPVYVFLASNKASYVTAQIYGVTGGEAINL 3TZQ Chain:B ((8-249)) -----------------------------------------------ELENKVAIITGACGGIGLETSRVLARAGARVVLADLP--ETDLAGAAASV---GRGAVHHVVDLTNEVSVRALIDFTIDTFGRLDIVDNNAAHSDPADMLVTQMTVDVWDDTFTVNARGTMLMCKYAIPRLISAGGGAIVNISSATAHAAYDMSTAYACTKAAIETLTRYVATQYGRHGVRCNAIAPGLVRTP-RLE---PQPIVDIFATHHLAGRIGEPHEIAELVCFLASDRAAFITGQVIAADSG-----

General information: TITO was launched using: RESULT: Template: 3TZQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1330 -100803 -75.79 -427.13 target 2D structure prediction score : 0.64 Monomeric hydrophicity matching model chain B : 0.75 3D Compatibility (PKB) : -75.79 2D Compatibility (Sec. Struct. Predict.) : 0.64 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.532

(partial model without unconserved sides chains): PDB file : Tito_3TZQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3TZQ-query.scw PDB file : Tito_Scwrl_3TZQ.pdb: