Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNKGGQMKKVMFAGLSLLSLVVLMACGEEETKKTQAAQQPKQQTTVQQISVGKDVPDFTLQSMDGKEVKLSDFKGKKVYLKFWASWCGPCKKSMPELMELAAKPDRDFEILTVIAPGIQGEKTVEQFPQWFQEQGYKDIPVLYDTKATTFQAYQIRSIPTEYLIDSQGKIGKIQFGAISNADAEAAFKEMN 5UM7 Chain:B ((46-186)) ----------------------------------------------EHELTGQQLPEFEMVDQAGYQKKSAEFYNKPMLVVEWASWCPDCQKQLPEIQKVYEKYKGKIHFVMLDMLDSK-RETKERADQYISEKDY-TFPYYYDTDERAADILHVQSIPTIYLVDKNQKVKKVMTDFHDEAALEKQLEE--

General information: TITO was launched using: RESULT: Template: 5UM7.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 674 -60350 -89.54 -428.01 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain B : 0.70 3D Compatibility (PKB) : -89.54 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.70 QMean score : 0.501

(partial model without unconserved sides chains): PDB file : Tito_5UM7.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UM7-query.scw PDB file : Tito_Scwrl_5UM7.pdb: