Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MRNEMMHRPVVKEELLDFMRTQQKKLPGELGKLEEEAHVAEVPVI---PHETVVFLKFLLGQLQPERILEIGAAIGFSSSVMATTI-GENAHVTTIDRFDVMIEKAKKNYERLGLTDKVTLLEGQAADILPELSGPYDFIFMDSAKSKYIEFLPECLR--LLRKGGVLMVDDIFQGGTILLP------DEEIPRGKRAIHRKLNEFLRVVMDHPDLTSTILPLGDGVILMTKESETIEL 4YMH Chain:B ((2-236)) LGSILPFNEETADRVSAYCEKNSHGIPDALVEHWEWTRTRFPDADKMSSRLQGSWMIFTARDRKPKRILEIGCYSGYSALAWYEGTRDTKAEIVTLEYSPKMIAASREAFKKYGVGDRVKLIEGPAENTLKTLEGEFDLIFVDANKDGYAGYVKTILDQGLLSANGIILCDNVFARGLTIGPDCAPWLNDHVRPYWNGCGQALDKFSAGLMEDPRIDVLLLPVFDGVTQIRWKDG----

General information: TITO was launched using: RESULT: Template: 4YMH.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 1041 -17337 -16.65 -77.74 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain B : 0.73 3D Compatibility (PKB) : -16.65 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.73 QMean score : 0.545

(partial model without unconserved sides chains): PDB file : Tito_4YMH.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4YMH-query.scw PDB file : Tito_Scwrl_4YMH.pdb: