Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDSEGFRPNVGIILAN---DDGQVLWAKRIG---HNAWQFPQGGIQF--GETPEQALFRELREEIGLLPEHVQIIAQTKGWLRYRLPHRYIRSDSDPVCIGQKQKWFLLKLTAPAKNIQLNLADPPEFDEWQWVSYWYPLGQVVNF-KRDVYRKAMVELCTQLPVQQLP 3GZ5 Chain:A ((21-169)) ------QLLTVDAVLFTYHDQQLKVLLVQRSNHPFLGLWGLPGGFIDETCDESLEQTVLRKLAEKTAVVPPYIEQLCTVGNN------SRDARG-------WSVTVCYTALMSYQAC--QIQ---IASVSDVKWWPLADVLQMPLAFDHLQLIEQARERLTQKALYSLV-

General information: TITO was launched using: RESULT: Template: 3GZ5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 656 -5393 -8.22 -39.65 target 2D structure prediction score : 0.54 Monomeric hydrophicity matching model chain A : 0.66 3D Compatibility (PKB) : -8.22 2D Compatibility (Sec. Struct. Predict.) : 0.54 1D Compatibility (Hydrophobicity) : 0.66 QMean score : 0.342

(partial model without unconserved sides chains): PDB file : Tito_3GZ5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3GZ5-query.scw PDB file : Tito_Scwrl_3GZ5.pdb: