Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MAYDDQNIFAKILRGELPAIKLYEDDQVLAFMDIMPQADGHALVIPKTPAVTLLDLPPEAAAYTIQIVQKIAKAMETALNLDGIVLMQLSGAAAGQTVPHVHFHLIPTNVHQLGKHAAQLGDQDKIKALAEKIKAAL 4ZGL Chain:D ((1-102)) -----MNVFEKIIQGEIPCSKILENERFLSFYDINPKAKVHALVIPKQSIQDFNGITPELMAQMTSFIFEVVEKLG--IKEKGYKLLTNVGKNAGQEVMHLHFHILSGD----------------------------

General information: TITO was launched using: RESULT: Template: 4ZGL.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 429 -79388 -185.05 -778.31 target 2D structure prediction score : 0.69 Monomeric hydrophicity matching model chain D : 0.75 3D Compatibility (PKB) : -185.05 2D Compatibility (Sec. Struct. Predict.) : 0.69 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.706

(partial model without unconserved sides chains): PDB file : Tito_4ZGL.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4ZGL-query.scw PDB file : Tito_Scwrl_4ZGL.pdb: