Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MTEERSLVERAKEYDASQI-QVLEGLEAVRKRPGMYIGSTS-SEGLHHLVWEIVDNSIDEVLAGFA--TKIHVIIEKD-NSITVIDDGRGIPVDIQAKTGRPAVETVFTVLHAGGKFGGGGYKVSG-------GLHGVGSSVVNALSTQLDVKVY--KEGNVYYQEYRRGAVVDDLKIIEQTDR--HGTTVHFT-------PDPEIFTETTEFDFSKLATRIRELAFLNRGMRISIEDKREEEPVINEYHYDGGIKSYVEYLNANKTVIFPEPVYLEGEQQDIAVEVSMQYTDGYHSNIMSFANNIHTYEGGTHESGFKTALTRVINDYARKQKLMKENDDNLTGEDVREGLTAVISIKHPDPQFEGQTKTKLGNSEVRTVTDRLFSEHFMKFLLENPSVGRQIVEKGLLASRARLAAKRAREVTRRKGALEISNLPGKLADCSSNDPEKCELFIVEGDSAGGSAKQGRNREFQAILPIRGKILNVEKASMDKILANEEIRSLFTAMGTGFGEDFDVSKARYHKLVIMTDADVDGAHIRTLLLTLFYRYMRPIVEAGYVYIAQPPLYGVKQGKNITYVQPGKNAEEELKQVVASLPASPKPSVQRYKGLGEMDDHQLWETTMDPSNRMMARVSVDDAIAADQIFEMLMGDRVEPRRAFIEENAHYVKNLDI 1EI1 Chain:A ((1-219)) ----------SNSSDSSSIK-VLKGLDAVRKRPGMYIGDTDDGTGLHHMVFEVVDNAIDEALAG--HCKEIIVTIHA-DNSVSVQDDGRGIPTGIHPEEGVSAAEVIMTVLHAGGKFDD-------NSYKVSGGLHGVGVSVVNALSQKLELVIQREGKIHRQIYE--HGVPQAPLAVTGET--EKTGTMVRFWPSLETFT-------NVTEFEYEILAKRLRELSFLDSGVSIRLRD-KRD--GKEDHFHYEG---------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 1EI1.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 941 16371 17.40 83.52 target 2D structure prediction score : 0.61 Monomeric hydrophicity matching model chain A : 0.62 3D Compatibility (PKB) : 17.40 2D Compatibility (Sec. Struct. Predict.) : 0.61 1D Compatibility (Hydrophobicity) : 0.62 QMean score : 0.525

(partial model without unconserved sides chains): PDB file : Tito_1EI1.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1EI1-query.scw PDB file : Tito_Scwrl_1EI1.pdb: