Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MIDSEGFRPNVGIILANDDGQVLWAKRIGHNAWQFPQGGIQFGETPEQALFRELREEIGLLPEHVQIIAQTKGWLRYRLPHRYIRSDSDPVCIGQKQKWFLLKLTAPAKNIQLNLADPPEFDEWQWVSYWYPLGQVVNF-KRDVYRKAMVELCTQLPVQQLP 4HFQ Chain:B ((68-195)) ------PLMDVRAWIVE-DEKICLVRGQGEDSWALPGGFGEVGYSPTENILKEIEEETGFKAKVERLLAVFDTNRFQ-LQ-----------SKQYTKFVFGCKLLDGQ--FQ----ENQEIADLQFFAIDQLPNLSEKRITKEQIELLWQVYQ---------

General information: TITO was launched using: RESULT: Template: 4HFQ.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain B - contact count / total energy / energy per contact / energy per residue : 638 -48436 -75.92 -381.38 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain B : 0.65 3D Compatibility (PKB) : -75.92 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.65 QMean score : 0.456

(partial model without unconserved sides chains): PDB file : Tito_4HFQ.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4HFQ-query.scw PDB file : Tito_Scwrl_4HFQ.pdb: