Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MKLEGYNNFYNSVLSLCTIGAFSYSSSNLGYGVSIGRYCSLASNIKIMGAH---HFTDWVSTSPHFYTEDYHNTDQALVTHNYRSRRNVTIGNDVWIGADVVLKNNITIGDGAIIASNSVITKNVEPYTIVGGNPARLIRKRFEDTVIKELTELKWWRFHKNDLKGLN----FIEPIDFIKNLQKRIYLEKLNEFFPPIFTKNDLLKSS 5UX9 Chain:D ((70-219)) -------------------------------KLRIGKFCSIASGATFMMAGNQGHRADWISTFPFSKKEF---GEG--VKDGFQRAGDTIVGNDVWIGSEAMIMPGVHIGDGAIIGARAVITKNVAPYSVVVGNNVVV-KKRFDENLIQTLLVIKWWDWPLQHIKNTMEILCSGHIEELEQYFIKNV----------------------

General information: TITO was launched using: RESULT: Template: 5UX9.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain D - contact count / total energy / energy per contact / energy per residue : 566 -7721 -13.64 -53.99 target 2D structure prediction score : 0.59 Monomeric hydrophicity matching model chain D : 0.68 3D Compatibility (PKB) : -13.64 2D Compatibility (Sec. Struct. Predict.) : 0.59 1D Compatibility (Hydrophobicity) : 0.68 QMean score : 0.475

(partial model without unconserved sides chains): PDB file : Tito_5UX9.pdb: (Unconserved sides chains are recalculated) : Sequence: align-5UX9-query.scw PDB file : Tito_Scwrl_5UX9.pdb: