Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MNRFASKEEEKLYYEHKASEEEFLNWYQKQERPEYDKPSLTVDIVLMCYNK--EEDQLKVLLIQRKG-------HPYRNSWALPGGFVQKDESTGESVLRETKEETGVVISKENIEQLHTFSTPNRDPRGWVVTVSYLAFIGEEP---LIAGDDAKEVRWFTLERHSNKIHLSSGEVAISLDLVTGESSGKDTLAFDHSQIILKAFNRVVNKMEHEPQVLQVLGKDFTITEARKVFAKFLGIDYKTIDHSNFKKAMLQYFDEIGERPVGIGRPSKIYQLKPGHHE 3I9X Chain:A ((27-177)) --------------------------------------GYTSDMILTTVKELNGKPTLHILLIKRSLTNAEGKPNMEGGKWAVPGGFVDENESAEQAAERELEEETSLTDI--PLIPFGVFDKPGRDPRGWIISRAFYAIVPPEALEKRAAGDDAAEIGLFPMTEALE-----------------------LPLAFDHLDMLKKAFSAITEEFL-----------------------------------------------------------------------

General information: TITO was launched using: RESULT: Template: 3I9X.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 632 17946 28.40 129.11 target 2D structure prediction score : 0.68 Monomeric hydrophicity matching model chain A : 0.69 3D Compatibility (PKB) : 28.40 2D Compatibility (Sec. Struct. Predict.) : 0.68 1D Compatibility (Hydrophobicity) : 0.69 QMean score : 0.589

(partial model without unconserved sides chains): PDB file : Tito_3I9X.pdb: (Unconserved sides chains are recalculated) : Sequence: align-3I9X-query.scw PDB file : Tito_Scwrl_3I9X.pdb: