Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence ----MRYRIHYIDVQNALQNYIWILEDTETHEAVAVDP-TEAELVTHFCQEHQ---LTLKQIWLTHWHKDHIGGVADLTANTP-MAVYGPRDELSKIP---GITHPLQHD-DHLKFNDLKVEIIATPGHTLGHIVYFIEEL-----NALFCGDTLFAMGCGRL-----FEGTAEQMYHSLNRLAA-LPTATKVYCTHEYTLSNAEFALSVEPENHALQERAEQVRMLRQEGKITLPSSIELELATNPFLRAE------------SVDEFAHLRSLKDNF----- 1QH5 Chain:A ((1-260)) MKVEVLPALT--------DNYMYLVIDDETKEAAIVDPV-QPQKVVDAARK-HGVK--LTTVLTTHHHWDHAGGNEKLVKLESGLKVYGGDD------RIGALTHKIT-HLSTLQVGSLNVKCLATPCHTSGHICYFVSKPGGSEPPAVFTGDTLFVA---GCGKFYEG--TADEMCKALLEVLGRLPPDTRVYCGHEYTINNLKFARHVEPGNAAIREKLAWAKEKYSIGEPTVPSTLAEEFTYNPFMRVREKTVQQHAGETDPVTTMRAVRREKDQFKMPRD

General information: TITO was launched using: RESULT: Template: 1QH5.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1252 10946 8.74 49.98 target 2D structure prediction score : 0.57 Monomeric hydrophicity matching model chain A : 0.75 3D Compatibility (PKB) : 8.74 2D Compatibility (Sec. Struct. Predict.) : 0.57 1D Compatibility (Hydrophobicity) : 0.75 QMean score : 0.493

(partial model without unconserved sides chains): PDB file : Tito_1QH5.pdb: (Unconserved sides chains are recalculated) : Sequence: align-1QH5-query.scw PDB file : Tito_Scwrl_1QH5.pdb: