Modeling by threading (Tito software) Unconserved sides chains calculation (Scwrl software) Evaluation (QMean software) Input alignment information: Query sequence MWVKDMFILGSGDQGLAVLSIYEG----EVEGLVDIFEEGRFIGCKILDKTVLGGLKWFESLLLTLDRKPRAIVAFGDNFKREEIFCSYK-NKVEYINVICNSARIFKHSFLGKGNFIGTNVTIQALVEIGDNNIINSGSIVSCNCKIGNNVNISPGVILSGNVKIDDNVFIGAGATIRDAVSIGFGAIIGAGATVIHNVPENAVVVGTPGKIIKYRSV 4M99 Chain:A ((3-208)) -GNRKLAVIGAGGHGKVVAELAAALGTYGEIVFLDDRTQ-----GSVNGFPVIGTTLLLENSLS--PEQFDITVAVGNNRIRRQITENAAALGFKLPVLIHPDATVSPSAIIGQGSVVMAKAVVQAGSVLKDGVIVNTAATVDHDCLLDAFVHISPGAHLSGNTRIGEESRIGTGACSRQQTTVGSGVTAGAGAVIVCDIPDGMTVAGNPAKPL-----

General information: TITO was launched using: RESULT: Template: 4M99.pdb Alignment : align.pir Tito was launched with SMD and SCWRL Tito text output Monomeric PKB - chain A - contact count / total energy / energy per contact / energy per residue : 1239 -36511 -29.47 -181.65 target 2D structure prediction score : 0.43 Monomeric hydrophicity matching model chain A : 0.71 3D Compatibility (PKB) : -29.47 2D Compatibility (Sec. Struct. Predict.) : 0.43 1D Compatibility (Hydrophobicity) : 0.71 QMean score : 0.484

(partial model without unconserved sides chains): PDB file : Tito_4M99.pdb: (Unconserved sides chains are recalculated) : Sequence: align-4M99-query.scw PDB file : Tito_Scwrl_4M99.pdb: